The last day!
Breakfast and packing, whilst trying to get over the "staying up until past 2:00am at the disco and possibly having imbibed a little more than one is use to" feeling.
Anyway, happily this is about feedback and control systems in the body, and we're looking at blood pressure and pulse rates. We start by measuring resting blood pressure and pulse rates. Then, given our state, we decide to test the hypothesis that they will decrease if we lie down for a few minutes. We all try that out, and then move on to other scenarios. Maybe caffeine in the coffee at coffee break has an effect, we test that out. Others look at exercise and also lying head down and standing on their heads and so on. Still feeling a little rough, we decide standing on ones head is probably going to end in a certain amount of disaster, so we continue with the lieing down theme.
I manage to sidetrack the tutor into a discussion on the comparative anatomy of the crocodilian three chambered heart, and how a giraffe manages its blood pressure. Then, a debriefing followed by a bit of theory on the heart and one of the people is hooked up to an ECG. We compare heart rates with breathing cycles and also the effect of holding your breath on the heart pumping mechanics. Finally, the tutor shows us the dive response by wiring himself up, and then plunging his face into a bowl of iced water! What a way to end!!
The final lunch, and then the final briefing to the whole school about the ECA and then we got the ECA and that was that!
Once you leave campus, the real world starts to filter back in and the residential school starts to seem more like a dream. For that week you are isolated from most of the world, I saw no TV, read no newspapers, and really had little idea what was going on outside the course (and was probably better for it!). So focused you are in the course, its hard to get much time for normal things. Some people attempted TMAs and things while there, but I don't think many were done.
With a typical day (for me anyway) starting with getting up at 7ish, walking into breakfast at about 7:45, chatting with others, then we assembled at about 8:30 for the walk to the QMC for a 9:00 start. Labs from 9-12:30 or so, lunch 12:30-1:30, labs 1:30-5, tea at 5:45, briefings and other sessions 7:00-9:00, bar from 9:00-whenever. Its a full day!
Friday, 27 July 2007
Thursday, 26 July 2007
SXR270 - day 6
Thursday - and its a new activity. Regulation and control.
We start off the morning looking at samples of blood glucose taken from various patients doing various things, including a type II diabetic. This is a lot of work with automatic pipettes and test tubes. After filling 48 tubes with various mixtures we then have to add detection enzymes and run the tubes through a colorimeter. This allows the amount of glucose to be recorded, once we have plotted a graph of controlled samples to calibrate the device. However with 48 different tubes and labels, its quite easy to get a bit confused on the 32nd or so and miss your place, so care is needed.
Its a fairly gentle exercise and takes us up to lunch.
After lunch we go into a different lab and start to play with Douglas bags. These are large plastic bags that will capture your exhaled air which can then be analysed and measured.
We start by using them to try and work out a basal metabolic rate. After we have this, we move onto looking at effects, such as exercise, caffeine intake, chocolate, smoking and so on.
This can then be compared against the base value and we can see the sort of effect it has on you.
After the day is done, there is the option to go and have a look at the level 3 labs and see what they do in those. It looks a little daunting in some respects, and you have to pass a test even before starting. However most people seem to be getting on with it ok.
Tea - then a free evening, and after a certain amount of internal debate I decided to get a disco ticket. We hung around in the Cripps bar until about 10ish, then wandered down to the portland building. People started arriving in significant numbers after a few minutes, and soon some brave souls, lead by the course director, were dancing.
We partied on into the night. Later on some more of the double-blues turned up, including jiving Jonathan. We all got into the spirit - in more ways than one by then. It all wound up at 1am, despite saying 9:30-1:30 on the ticket. One or two of our party went and had a quiet word with the DJ while the rest of us ran for cover fearing the fallout.
We retired back to hall and continued for a while trying to keep quiet in a room. The smokers finally broke rank and I went to bed just after 2:00am.
We start off the morning looking at samples of blood glucose taken from various patients doing various things, including a type II diabetic. This is a lot of work with automatic pipettes and test tubes. After filling 48 tubes with various mixtures we then have to add detection enzymes and run the tubes through a colorimeter. This allows the amount of glucose to be recorded, once we have plotted a graph of controlled samples to calibrate the device. However with 48 different tubes and labels, its quite easy to get a bit confused on the 32nd or so and miss your place, so care is needed.
Its a fairly gentle exercise and takes us up to lunch.
After lunch we go into a different lab and start to play with Douglas bags. These are large plastic bags that will capture your exhaled air which can then be analysed and measured.
We start by using them to try and work out a basal metabolic rate. After we have this, we move onto looking at effects, such as exercise, caffeine intake, chocolate, smoking and so on.
This can then be compared against the base value and we can see the sort of effect it has on you.
After the day is done, there is the option to go and have a look at the level 3 labs and see what they do in those. It looks a little daunting in some respects, and you have to pass a test even before starting. However most people seem to be getting on with it ok.
Tea - then a free evening, and after a certain amount of internal debate I decided to get a disco ticket. We hung around in the Cripps bar until about 10ish, then wandered down to the portland building. People started arriving in significant numbers after a few minutes, and soon some brave souls, lead by the course director, were dancing.
We partied on into the night. Later on some more of the double-blues turned up, including jiving Jonathan. We all got into the spirit - in more ways than one by then. It all wound up at 1am, despite saying 9:30-1:30 on the ticket. One or two of our party went and had a quiet word with the DJ while the rest of us ran for cover fearing the fallout.
We retired back to hall and continued for a while trying to keep quiet in a room. The smokers finally broke rank and I went to bed just after 2:00am.
Labels:
residential,
sxr270
Wednesday, 25 July 2007
SXR270 - day 5
Wow - those days keep rolling by!
We started this morning after breakfast in the plant lab again. This time we ground up some plant leaves to extract the chloroplasts. This involved a pestle and mortar, an extraction buffer, a couple of trips to the centrifuge, and quite a lot of ice to keep them from degrading. It was also our first encounter with whizzy stirrers.
Once we had prepared the extraction, we used DCTIP to check that we had captured chloroplasts and that things were working. This is a die that is blue, but turns colourless as it hijacks the electrons produced from the photosystem electron transfer chains. Extremely convenient as it shows you photosynthesis in action.
First we measured the degradation of the die with white light. Then we tried a few different filters to see the effect red, green, blue, orange and yellow light had on the rate of photosynthesis.
Plotting these on a graph showed the light colours that were best absorbed with the steepest lines.
Red and blue are favourites, green not very good and orange, yellow and light somewhere in between. So that's why plants are green - they absorb the rest.
Other groups tried light intensity variations which showed an increase with increasing light - up to a point. Photooxidation then starts to kick in as the leaves get more light than they can handle. We have a debrief on this session which ends with Rachel posing us the question "why aren't plant leaves black and so absorbing all light wavelengths?"
Lunch was followed by a poster session. We had two hours to make a scientific poster on any of the activities we had done with plants. I did mine on the results from yesterdays lime leaves which gave some rather fine data. As usual, for me, I had about finished it after 30 minutes, but then all the little touching up and fiddling around and reprinting took another hour or so. I asked Rachel why plants are green, to which she suggested it was to do with not absorbing too much light and so causing overheating.
Eventually my poster was as done as it was ever going to be, and it was framed in a big sleeve and pinned up on a set of display boards.
I went back to Rachel unconvinced about the black/green plant leaf explanation, challenging her on plants in low light environments like forest floors and Arctic tundra. Finally I found something she couldn't answer! Boy that was hard work, she had an answer for everything else!!
Then back to the room to crash out for a while until tea, then its back to the QMC to do poster viewing.
We looked and commented on our own posters after a break, and also at all the others in the group. Then we went on to look at the other groups and see what they looked like. It was quite useful in some ways. You got to see posters with bits in that you thought you'd done better. You also saw some ideas you wish you'd have thought of too. Still - in the end I was satisfied with my efforts, and the feedback was fairly good too. I made some rather light hearted remarks on the feedback form, not realising they would be collected in. I hope it doesn't count against me!!
We decided to go to Lenton bar tonight and had a few drinks there. After we returned I thought I'd just poke my nose into Cripps bar to see what was going on, and somehow got into a discussion on Tim's view of exactly what risk assessment means to him (everything apparently!).
We started this morning after breakfast in the plant lab again. This time we ground up some plant leaves to extract the chloroplasts. This involved a pestle and mortar, an extraction buffer, a couple of trips to the centrifuge, and quite a lot of ice to keep them from degrading. It was also our first encounter with whizzy stirrers.
Once we had prepared the extraction, we used DCTIP to check that we had captured chloroplasts and that things were working. This is a die that is blue, but turns colourless as it hijacks the electrons produced from the photosystem electron transfer chains. Extremely convenient as it shows you photosynthesis in action.
First we measured the degradation of the die with white light. Then we tried a few different filters to see the effect red, green, blue, orange and yellow light had on the rate of photosynthesis.
Plotting these on a graph showed the light colours that were best absorbed with the steepest lines.
Red and blue are favourites, green not very good and orange, yellow and light somewhere in between. So that's why plants are green - they absorb the rest.
Other groups tried light intensity variations which showed an increase with increasing light - up to a point. Photooxidation then starts to kick in as the leaves get more light than they can handle. We have a debrief on this session which ends with Rachel posing us the question "why aren't plant leaves black and so absorbing all light wavelengths?"
Lunch was followed by a poster session. We had two hours to make a scientific poster on any of the activities we had done with plants. I did mine on the results from yesterdays lime leaves which gave some rather fine data. As usual, for me, I had about finished it after 30 minutes, but then all the little touching up and fiddling around and reprinting took another hour or so. I asked Rachel why plants are green, to which she suggested it was to do with not absorbing too much light and so causing overheating.
Eventually my poster was as done as it was ever going to be, and it was framed in a big sleeve and pinned up on a set of display boards.
I went back to Rachel unconvinced about the black/green plant leaf explanation, challenging her on plants in low light environments like forest floors and Arctic tundra. Finally I found something she couldn't answer! Boy that was hard work, she had an answer for everything else!!
Then back to the room to crash out for a while until tea, then its back to the QMC to do poster viewing.
We looked and commented on our own posters after a break, and also at all the others in the group. Then we went on to look at the other groups and see what they looked like. It was quite useful in some ways. You got to see posters with bits in that you thought you'd done better. You also saw some ideas you wish you'd have thought of too. Still - in the end I was satisfied with my efforts, and the feedback was fairly good too. I made some rather light hearted remarks on the feedback form, not realising they would be collected in. I hope it doesn't count against me!!
We decided to go to Lenton bar tonight and had a few drinks there. After we returned I thought I'd just poke my nose into Cripps bar to see what was going on, and somehow got into a discussion on Tim's view of exactly what risk assessment means to him (everything apparently!).
Labels:
residential,
sxr270
Tuesday, 24 July 2007
SXR270 - day 4
Day four - and we move on to theme 3, the plants one.
After the briefing the night before, we were urged to find some interesting plant leaves from anywhere we could to compare carbon uptake. This is a recurring idea in the course. Unlike SXR103 where you sort of just follow a script, here you have to make your own hypotheses and can even devise your own experiments within certain boundaries.
Anyway, Cheryl had this great idea to find some holly and some ivy, and then we could make a bit of a theme if we wrote it up as a poster. However it took a while to track down some ivy. We eventually found some half way to the lab.
In the lab after a briefing we decided to subject the variegated holly to the carbon 14 test. We picked bits of the leaf that were yellow to compare with green bits. After a few background counts we subjected the leaf discs to about 20 mins of 14C exposure, and then started to look at the results with a GM tube. The discs we had cut were quite small so the results were not much above background rates. Background was around 30, and we were getting 50-60 on the green bits. The t-test showed these results as significant.
After this, we tried some lime leafs - one of which was growing in sunlight, the other in shade. This was far more dramatic. The shaded leafs gave counts of 3600, and those in the light around 700. After we did the maths the results were highly significant. Some good results!
We finished up the morning by looking at leaf impressions that showed the stomata under a microscope to see if we could see the structures.
So the holly and the ivy theme sort of disappeared.
After lunch, we moved onto stomatal control, looking at the effects of light, dark, CO2 and ABA on the opening of stomata. We had to peal epidermal layers off a leaf and then subject them to the various conditions. Results were rather disappointing not really leading to very solid conclusions. Some of them went the way the text book and tutors expected, but with quite small margins. Others went the opposite way. We weren't really clear what went wrong, or if we had discovered something new! However it seemed it wasn't the first time this had happened.
The tea, and a debrief on the days experiments, followed by a briefing on the next days experiment. Then a session roughing out a design for a scientific poster to be done tomorrow. Rachel the activity tutor was like a walking encyclopaedia of latin names for plants. She just kept reeling them off.
Then to the bar for further discussion!
After the briefing the night before, we were urged to find some interesting plant leaves from anywhere we could to compare carbon uptake. This is a recurring idea in the course. Unlike SXR103 where you sort of just follow a script, here you have to make your own hypotheses and can even devise your own experiments within certain boundaries.
Anyway, Cheryl had this great idea to find some holly and some ivy, and then we could make a bit of a theme if we wrote it up as a poster. However it took a while to track down some ivy. We eventually found some half way to the lab.
In the lab after a briefing we decided to subject the variegated holly to the carbon 14 test. We picked bits of the leaf that were yellow to compare with green bits. After a few background counts we subjected the leaf discs to about 20 mins of 14C exposure, and then started to look at the results with a GM tube. The discs we had cut were quite small so the results were not much above background rates. Background was around 30, and we were getting 50-60 on the green bits. The t-test showed these results as significant.
After this, we tried some lime leafs - one of which was growing in sunlight, the other in shade. This was far more dramatic. The shaded leafs gave counts of 3600, and those in the light around 700. After we did the maths the results were highly significant. Some good results!
We finished up the morning by looking at leaf impressions that showed the stomata under a microscope to see if we could see the structures.
So the holly and the ivy theme sort of disappeared.
After lunch, we moved onto stomatal control, looking at the effects of light, dark, CO2 and ABA on the opening of stomata. We had to peal epidermal layers off a leaf and then subject them to the various conditions. Results were rather disappointing not really leading to very solid conclusions. Some of them went the way the text book and tutors expected, but with quite small margins. Others went the opposite way. We weren't really clear what went wrong, or if we had discovered something new! However it seemed it wasn't the first time this had happened.
The tea, and a debrief on the days experiments, followed by a briefing on the next days experiment. Then a session roughing out a design for a scientific poster to be done tomorrow. Rachel the activity tutor was like a walking encyclopaedia of latin names for plants. She just kept reeling them off.
Then to the bar for further discussion!
Labels:
residential,
sxr270
Monday, 23 July 2007
SXR270 - day 3
Day three starts with us in breakfast consoling ourselves about not winning in the quiz last night.
Then - after the walk to the QMC, we're back in the lab, this time looking at cells from manduca sexta.
They eat a diet rich in potassium, so they get a lot of K+ in their diet, which they have to manage. A lot of this enters their system, and they have to get it out again before it messes up their internals.
We hypothesised that there was some sort of active transport going on here, to pump the ions back out.
We started our day by looking at the pH values of the various stages of the gut, food and faeces of the worm, and also its internal hemolymph system. This is because one hypothesis is that there is a H+/K+ antiporter. If this is the case then the pH should change as protons are pumped in exchange for K+ ions.
We then went on to measure electrical potential across the gut wall using a small piece of tissue held in a clamp between two solutions. We altered the potassium concentration to try and see what rate it could pump the ions, and we also used an electron transport chain blocking substance dinitrophenol to disable ATP synthesis and to see if that stopped the pumping and so gave evidence for an energy hungry transport reaction.
Another group look at sections of tissue under the microscope and also electron-micrographs to look for clues in the histology of the cells.
After lunch we had to prepare a five minute presentation with slides about some aspect of the work we had done over the past two days. Sue and I did a presentation on oxygen uptake differences between skin and muscle cells based on the data we found yesterday.
Others did similar sorts of things, or ones based around the manduca investigation. There was a lot of good stuff there, but with the best will in the world, by the time you'd heard the 10th presentation on oxygen uptake in muscle v. liver, it began to pale a little.
Once that was done and we'd listened to all the talks, we went back for tea, just beating the rain.
After tea, a briefing on the next days activity, photosynthesis. We also had a partner shuffle.
This was followed by a poster session where we had 20 minutes to make a poster based on a rather sketchy experimental report. We did it in 10! That probably wasn't the point though!
A night in the bar listening (and avoiding) to the karaoke, but chatting with our next lab partners and some friends.
A pretty early night really.
Then - after the walk to the QMC, we're back in the lab, this time looking at cells from manduca sexta.
They eat a diet rich in potassium, so they get a lot of K+ in their diet, which they have to manage. A lot of this enters their system, and they have to get it out again before it messes up their internals.
We hypothesised that there was some sort of active transport going on here, to pump the ions back out.
We started our day by looking at the pH values of the various stages of the gut, food and faeces of the worm, and also its internal hemolymph system. This is because one hypothesis is that there is a H+/K+ antiporter. If this is the case then the pH should change as protons are pumped in exchange for K+ ions.
We then went on to measure electrical potential across the gut wall using a small piece of tissue held in a clamp between two solutions. We altered the potassium concentration to try and see what rate it could pump the ions, and we also used an electron transport chain blocking substance dinitrophenol to disable ATP synthesis and to see if that stopped the pumping and so gave evidence for an energy hungry transport reaction.
Another group look at sections of tissue under the microscope and also electron-micrographs to look for clues in the histology of the cells.
After lunch we had to prepare a five minute presentation with slides about some aspect of the work we had done over the past two days. Sue and I did a presentation on oxygen uptake differences between skin and muscle cells based on the data we found yesterday.
Others did similar sorts of things, or ones based around the manduca investigation. There was a lot of good stuff there, but with the best will in the world, by the time you'd heard the 10th presentation on oxygen uptake in muscle v. liver, it began to pale a little.
Once that was done and we'd listened to all the talks, we went back for tea, just beating the rain.
After tea, a briefing on the next days activity, photosynthesis. We also had a partner shuffle.
This was followed by a poster session where we had 20 minutes to make a poster based on a rather sketchy experimental report. We did it in 10! That probably wasn't the point though!
A night in the bar listening (and avoiding) to the karaoke, but chatting with our next lab partners and some friends.
A pretty early night really.
Labels:
residential,
sxr270
Sunday, 22 July 2007
SXR270 - day 2
Its day two on SXR270 - the day we get to do some real lab work.
It starts early. One of the discoveries at about 6am is how much a closing door echoes around the halls of residence. It appears we have an early riser in our block, so most of us awoke at around 6, and then tried to snooze in to a more reasonable hour.
After breakfast and a walk down to the QMC, we find our lab. Here Penny takes us through a pilot experiment that allows us to measure the oxygen update of a small piece of liver. This part is all about learning how to use the equipment, getting the procedure right, and learning how to interpret the results. The procedures are not too bad really - the data is all recorded on a computer which draws a graph of oxygen levels.
We basically leave it to respire for a while until it has used up all its glucose supply. Then we add some glucose solution which kicks off the respiration again. Then a bit later we give it a metabolic poison - potassium cyanide, which tends to stop it in its tracks.
That takes up the morning, together with some data analysis.
In the afternoon we start designing our own experiment. We chose to do rat skeletal muscle compared to rat skin. We figured the skin has very little metabolic activity and the muscle a fair bit. As it turned out the results weren't that different, although it was significant after running 5 replicates of each. Muscle really only uses oxygen quickly when its working, and this bit of muscle was just sitting around in a warm bath of buffer solution.
We spent a while interpreting our results and trying to work out if they were significant. We didn't have enough data for a t-test so we had to just compare things on a graph.
It looked ok, and we think we can make a convincing presentation of it in the 5 minute spot tomorrow.
After tea, we had more briefings and tutorials, which covered presenting the work tomorrow and what we shall be doing tomorrow in the experimental work in the morning. We get to meet a manduca hornworm catepillar and various bits of its guts.
We then proceed to see what its doing with potassium ions.
However that's another day, and tonight we have a quiz team to put together and win a quiz - or failing that at least not embarass ourselves too much!
Well what can I say? Our combined knowledge of cars seems to have been lacking, we didn't know who was the most popular children's author (no - we thought JK too - but its Beatrix Potter). It also took quite a while - we got there at 9:30, there were 30 questions and it didn't finish until nearly midnight. This is after we'd talked Cate into attending with the line "Well how long can it last?". Even Marks mobile phone tune I.D. gizmo didn't help. Still it was a good evening for getting to know people.
It starts early. One of the discoveries at about 6am is how much a closing door echoes around the halls of residence. It appears we have an early riser in our block, so most of us awoke at around 6, and then tried to snooze in to a more reasonable hour.
After breakfast and a walk down to the QMC, we find our lab. Here Penny takes us through a pilot experiment that allows us to measure the oxygen update of a small piece of liver. This part is all about learning how to use the equipment, getting the procedure right, and learning how to interpret the results. The procedures are not too bad really - the data is all recorded on a computer which draws a graph of oxygen levels.
We basically leave it to respire for a while until it has used up all its glucose supply. Then we add some glucose solution which kicks off the respiration again. Then a bit later we give it a metabolic poison - potassium cyanide, which tends to stop it in its tracks.
That takes up the morning, together with some data analysis.
In the afternoon we start designing our own experiment. We chose to do rat skeletal muscle compared to rat skin. We figured the skin has very little metabolic activity and the muscle a fair bit. As it turned out the results weren't that different, although it was significant after running 5 replicates of each. Muscle really only uses oxygen quickly when its working, and this bit of muscle was just sitting around in a warm bath of buffer solution.
We spent a while interpreting our results and trying to work out if they were significant. We didn't have enough data for a t-test so we had to just compare things on a graph.
It looked ok, and we think we can make a convincing presentation of it in the 5 minute spot tomorrow.
After tea, we had more briefings and tutorials, which covered presenting the work tomorrow and what we shall be doing tomorrow in the experimental work in the morning. We get to meet a manduca hornworm catepillar and various bits of its guts.
We then proceed to see what its doing with potassium ions.
However that's another day, and tonight we have a quiz team to put together and win a quiz - or failing that at least not embarass ourselves too much!
Well what can I say? Our combined knowledge of cars seems to have been lacking, we didn't know who was the most popular children's author (no - we thought JK too - but its Beatrix Potter). It also took quite a while - we got there at 9:30, there were 30 questions and it didn't finish until nearly midnight. This is after we'd talked Cate into attending with the line "Well how long can it last?". Even Marks mobile phone tune I.D. gizmo didn't help. Still it was a good evening for getting to know people.
Labels:
residential,
sxr270
Saturday, 21 July 2007
SXR270 - day1
Its daaaay 1 in the SXR270 residential house.
I arrived at about 11:30 and got myself registered. The family had come with me to see what it was like. It was all a bit weird. I was in Cripps Hall where I spent 3 years as an undergraduate. What's more, I'm in D-block, which was where I lived, and I was almost in the same room!
Things haven't changed much in the past 20 or so years either. The rooms look the same, the doors now have keycodes on the outside but otherwise it all looked very familiar.
We decided to take lunch in the dinning hall at Cripps and that hadn't changed much either!
Even the bar looked much the same, though it now had TV's in it.
After the family left, I had a little time to get my bearings and a cup of coffee then it was off to the medical school, a 10-15 min walk to have the introductory briefing. After that we went with our group and activity tutors to the lab we would be starting in. The group was spilt into two so we had a group tutor each - Rachel for us. We also gained a second dot at this point to distinguish the two subgroups. After we'd had a few things explained we had to introduce ourselves and get to know out colleagues a bit.
Then back to hall for tea.
After tea, we had a briefing on experiments, hypotheses and then on energy production and experimental procedure. It all went pretty well and the S204 material really helped understand the details. So tomorrow we are going to look at ATP production in various cell types and see if we can tell the difference between ATP production in muscle and skin based on oxygen consumption.
Retire to the bar for a drink or two and a bit of socialising. Day finished!
I arrived at about 11:30 and got myself registered. The family had come with me to see what it was like. It was all a bit weird. I was in Cripps Hall where I spent 3 years as an undergraduate. What's more, I'm in D-block, which was where I lived, and I was almost in the same room!
Things haven't changed much in the past 20 or so years either. The rooms look the same, the doors now have keycodes on the outside but otherwise it all looked very familiar.
We decided to take lunch in the dinning hall at Cripps and that hadn't changed much either!
Even the bar looked much the same, though it now had TV's in it.
After the family left, I had a little time to get my bearings and a cup of coffee then it was off to the medical school, a 10-15 min walk to have the introductory briefing. After that we went with our group and activity tutors to the lab we would be starting in. The group was spilt into two so we had a group tutor each - Rachel for us. We also gained a second dot at this point to distinguish the two subgroups. After we'd had a few things explained we had to introduce ourselves and get to know out colleagues a bit.
Then back to hall for tea.
After tea, we had a briefing on experiments, hypotheses and then on energy production and experimental procedure. It all went pretty well and the S204 material really helped understand the details. So tomorrow we are going to look at ATP production in various cell types and see if we can tell the difference between ATP production in muscle and skin based on oxygen consumption.
Retire to the bar for a drink or two and a bit of socialising. Day finished!
Labels:
residential,
sxr270
Thursday, 19 July 2007
SXR103 revisited
I had such a good time at SXR103 in 2006, it seemed a shame that you couldn't do it all over again. However after I'd written my diary, and the Open University had used it for their web site, I inquired what it took to become a tutor. It seems you need a degree in a relevant field (OK - I have one), a lot of enthusiasm, a sense of humour, and some teaching experience.
Well I didn't really have the latter, but I hoped I would have enough of the former to be considered, so I put in an application to be a tutor at the beginning of the year. You have to apply each year as the time comes around.
I thought nothing more of it, and approached it with a sense of "Nothing ventured, Nothing gained". What's the worst they could do?
Some months later, when I had completely forgotten about the application, I received a note in the post, saying I was allocated to the reserve list for a week at Sussex University and for this I would get a small fee. I was quite pleased, I mean at least they were willing to consider me, and that I wasn't beyond the pale! It did come as a slight surprise as I'd already heard other people on the bulletin board telling students that they would be tutoring for this week or that, so I assumed it was all past.
I talked to the people at work, and ended up booking the requisite week off, just in case, so I would be able to go. If nothing came of it, I'd just go to work as normal.
The day crept ever closer, and I heard nothing further, and then finally it was the start of "my" week. It was a long shot, one of the existing tutors falling ill of having problems for that week, so I wasn't really surprised I heard nothing.
Tea time on the Saturday and just getting tea ready with my wife for the children, and the phone rang. It was a number I'd not seen before - so probably a double glazing salesman. But no, it was Dianne the course director for the week. A tutor had to go home, could I get there for tomorrow and tutor activity A? To say I was shocked was an understatement. The words wind-up did come to mind. I said I thought I could make it, and hung up in a daze.
The rest of the night was a bit of a blur. I looked out my last years course stuff, got some clothes together. I tried to remember what the tutors dressed in - I was hoping smartish jeans and t-shirt would be ok as I'd be wearing a lab coat when on duty. Dianne had said I'd get a briefing from the senior tutor on the Monday, where I could ask questions. I assumed as I was covering that the other tutors would be doing the main work, and I'd be an assistant to help out.
I talked to Andy a bit, the senior tutor of activity A, and we agreed to meet up at 10:00 the following morning in the lab to just go over the experiment. Dianne then asked me if I had any tutorials I would like to give in the evenings. I hadn't really expected that, but I said I'd think about it (feverishly thinking I had no overheads, no computer and no ideas!). Andy mentioned over tea he was running a Maths help workshop/tutorial which I could help with.
We went to get some tea, and I thought a bit more on the tutorials. I suddenly thought I could write one on how life began almost from memory if I could get a computer. I went to see Barbara in the office to ask if there were any available computers. She took me over to the Pevensey building and gave me a login for one of them. I set to work. Luckily I had my pen drive in my pocket, and whilst browsing through it, found a powerpoint for an evolution talk I'd given a few months ago. Just the thing! It also had a collection of notes on it for the beginning of life I'd written last year. Things were really looking up. I made some adjustments to the evolution for the current audience, and roughed out a plan for the other one.
After I came out I ran almost immediately into Dianne and Barbara, so I told them I had two talks for them. Dianne said I'd do better with a more interesting title, so I changed it from evolution to "From Monkeys to Men" but stuck with the other one.
It was quiz night, and I joined an illegal tutors team (tutors weren't suppose to form teams with each other!). We all agreed it was a bit dire as quiz's go, but it helped me meet up with some of the tutors and get a bit more into the spirit of the thing. They turned out to be a really nice bunch of people.
I arrived at the lab early and re-familiarised myself with the equipment. Andy came in and we went through a few things. I watched a safety video and played around a bit with the experiment.
The rest of the day I was notionally helping out with Activity-E, but there were few questions and I managed to do a fair bit on my tutorials. Tea and then the bar after sitting in on a couple of tutorials. When the bar shut at about 11, Diane lead us to another bar which was open until 1am, where we talked into the night.
The morning session went pretty well - I was quick to admit my ignorance about rocks when pushed beyond the material that was provided in the notes and what I knew form S103. Usually this was a quick call to Nick who had a ready answer. I was quite relaxed by the time we got to lunch.
After lunch I watched carefully the demonstration to half the group, then repeated it later to the 2nd half with a few of my own additions. I forgot a couple of minor bits but it went ok. Nick told me I probably needed to speak a little louder.
I was more confident in the afternoon as I was fairly sure I could answer any tricky questions on physics. Andy suggested tomorrow I could do the final briefing, so I watched that carefully too.
My the end of the day my feet were hurting a little. There is no chance to sit down during the labs as you make a round of the students helping out.
The rest of the lab went well and we had all finished by 5ish. We went off to the tutors debrief and then to Tea. I attended a tutorial, and then gave one of my own - about 5-6 people attended. I think it went ok. A night in the slopes bar followed, but only until 11.
I don't think that went too well - if I did this session another year, I think I would write my own slides.
After tea I went to help Andy out with a maths problem class - which with about 11 people present it was just as well, and then to a drugs tutorial.
An evening in the slopes bar, followed by another night in the 1am bar! I can't remember much of it, except Nick was really on form, and my cheeks hurt from laughing the following morning.
After tea it was time for my other tutorial, which went pretty well too. After that it was back to the tutors room to have a few drinks before entry to the disco at about 11pm. We stayed there until it finished at 2am, and then one of the tutors who had not been drinking insisted we find a room and drink some wine. She disappeared after a whip-round to the local all-night ASDA and we found a quiet room to sit and drink until 4am.
I drove out of the campus, but my adventure was not over. Just as I got onto the main road, I found all was not right with the car, and pulled over. Just as I stopped one of the front tyres burst. It appears part of the suspension had somehow broken and dug into the inside wall of the tyre. So I had to call a recovery service, and get home on the train.
However - despite this rather miserable ending, I think I may be applying to teach again next year. Its every bit as much fun as doing the course.
Well I didn't really have the latter, but I hoped I would have enough of the former to be considered, so I put in an application to be a tutor at the beginning of the year. You have to apply each year as the time comes around.
I thought nothing more of it, and approached it with a sense of "Nothing ventured, Nothing gained". What's the worst they could do?
Some months later, when I had completely forgotten about the application, I received a note in the post, saying I was allocated to the reserve list for a week at Sussex University and for this I would get a small fee. I was quite pleased, I mean at least they were willing to consider me, and that I wasn't beyond the pale! It did come as a slight surprise as I'd already heard other people on the bulletin board telling students that they would be tutoring for this week or that, so I assumed it was all past.
I talked to the people at work, and ended up booking the requisite week off, just in case, so I would be able to go. If nothing came of it, I'd just go to work as normal.
The day crept ever closer, and I heard nothing further, and then finally it was the start of "my" week. It was a long shot, one of the existing tutors falling ill of having problems for that week, so I wasn't really surprised I heard nothing.
Tea time on the Saturday and just getting tea ready with my wife for the children, and the phone rang. It was a number I'd not seen before - so probably a double glazing salesman. But no, it was Dianne the course director for the week. A tutor had to go home, could I get there for tomorrow and tutor activity A? To say I was shocked was an understatement. The words wind-up did come to mind. I said I thought I could make it, and hung up in a daze.
The rest of the night was a bit of a blur. I looked out my last years course stuff, got some clothes together. I tried to remember what the tutors dressed in - I was hoping smartish jeans and t-shirt would be ok as I'd be wearing a lab coat when on duty. Dianne had said I'd get a briefing from the senior tutor on the Monday, where I could ask questions. I assumed as I was covering that the other tutors would be doing the main work, and I'd be an assistant to help out.
Sunday
I left Nottingham at about midday, and immediately got caught in traffic leaving the city where there were some major roadworks. Fuming slightly, I sat in a jam for about 45 minutes where they were repairing a bridge. Once I got past that, I got onto the motorway, and the rest of the journey was OK, if a little long. I managed to get to Sussex University for about 4:30, parked, and after following signs here and there posted around managed to find the OU offices. They had a badge ready for me, a room and some papers to sign. I then went and found my room and at just after 5, went to the course directors office to meet up with Dianne and the rest of the tutors. It was slightly nerve racking meeting so many people, and I was glad of the name badges as names are not my strong point. I joined in the debriefing session for the days activity and was introduced to all.I talked to Andy a bit, the senior tutor of activity A, and we agreed to meet up at 10:00 the following morning in the lab to just go over the experiment. Dianne then asked me if I had any tutorials I would like to give in the evenings. I hadn't really expected that, but I said I'd think about it (feverishly thinking I had no overheads, no computer and no ideas!). Andy mentioned over tea he was running a Maths help workshop/tutorial which I could help with.
We went to get some tea, and I thought a bit more on the tutorials. I suddenly thought I could write one on how life began almost from memory if I could get a computer. I went to see Barbara in the office to ask if there were any available computers. She took me over to the Pevensey building and gave me a login for one of them. I set to work. Luckily I had my pen drive in my pocket, and whilst browsing through it, found a powerpoint for an evolution talk I'd given a few months ago. Just the thing! It also had a collection of notes on it for the beginning of life I'd written last year. Things were really looking up. I made some adjustments to the evolution for the current audience, and roughed out a plan for the other one.
After I came out I ran almost immediately into Dianne and Barbara, so I told them I had two talks for them. Dianne said I'd do better with a more interesting title, so I changed it from evolution to "From Monkeys to Men" but stuck with the other one.
It was quiz night, and I joined an illegal tutors team (tutors weren't suppose to form teams with each other!). We all agreed it was a bit dire as quiz's go, but it helped me meet up with some of the tutors and get a bit more into the spirit of the thing. They turned out to be a really nice bunch of people.
Monday
Next morning I read through the tutor notes for the activity, and read my old copy of activity A worksheet. I called in on the computers on my way to the lab and did a bit more on the tutorials.I arrived at the lab early and re-familiarised myself with the equipment. Andy came in and we went through a few things. I watched a safety video and played around a bit with the experiment.
The rest of the day I was notionally helping out with Activity-E, but there were few questions and I managed to do a fair bit on my tutorials. Tea and then the bar after sitting in on a couple of tutorials. When the bar shut at about 11, Diane lead us to another bar which was open until 1am, where we talked into the night.
Tuesday
I woke early on Tuesday, despite the rather late night. Had breakfast then went to the lab early to have a final run through. Andy asked me what I'd like to do as far as demonstrations and briefings. I agreed to do the 2nd experimental briefing so I had time to watch carefully what he did.The morning session went pretty well - I was quick to admit my ignorance about rocks when pushed beyond the material that was provided in the notes and what I knew form S103. Usually this was a quick call to Nick who had a ready answer. I was quite relaxed by the time we got to lunch.
After lunch I watched carefully the demonstration to half the group, then repeated it later to the 2nd half with a few of my own additions. I forgot a couple of minor bits but it went ok. Nick told me I probably needed to speak a little louder.
I was more confident in the afternoon as I was fairly sure I could answer any tricky questions on physics. Andy suggested tomorrow I could do the final briefing, so I watched that carefully too.
My the end of the day my feet were hurting a little. There is no chance to sit down during the labs as you make a round of the students helping out.
The rest of the lab went well and we had all finished by 5ish. We went off to the tutors debrief and then to Tea. I attended a tutorial, and then gave one of my own - about 5-6 people attended. I think it went ok. A night in the slopes bar followed, but only until 11.
Wednesday
Another early start, breakfast and lab. This time I was more confident on the geology, and sailed through the morning mostly. I did the 2nd demonstration again, and finished the day with a debriefing session which is rather dry material and rather maths heavy for that time of the day.I don't think that went too well - if I did this session another year, I think I would write my own slides.
After tea I went to help Andy out with a maths problem class - which with about 11 people present it was just as well, and then to a drugs tutorial.
An evening in the slopes bar, followed by another night in the 1am bar! I can't remember much of it, except Nick was really on form, and my cheeks hurt from laughing the following morning.
Thursday
Much like Wednesday really, but it went a little more smoothly for me. I knew what I was doing by now and could also keep an eye on students progress with respect to time.After tea it was time for my other tutorial, which went pretty well too. After that it was back to the tutors room to have a few drinks before entry to the disco at about 11pm. We stayed there until it finished at 2am, and then one of the tutors who had not been drinking insisted we find a room and drink some wine. She disappeared after a whip-round to the local all-night ASDA and we found a quiet room to sit and drink until 4am.
Friday
I still woke at about 7:30, despite the short night. In breakfast the rest gradually filtered in. Us activity tutors were now finished, so we said our good byes after breakfast.I drove out of the campus, but my adventure was not over. Just as I got onto the main road, I found all was not right with the car, and pulled over. Just as I stopped one of the front tyres burst. It appears part of the suspension had somehow broken and dug into the inside wall of the tyre. So I had to call a recovery service, and get home on the train.
However - despite this rather miserable ending, I think I may be applying to teach again next year. Its every bit as much fun as doing the course.
Labels:
residential,
sxr103
Saturday, 7 July 2007
S204: TMA-4
Time to approach TMA-4. I must say the first time I looked at this it looked like gibberish! The essay question didn't look too bad, but the second question seemed impossible.
For this you have the books, but also a scientific paper was included attached to the question which summarised some of the different extremophiles. Extremophiles are organisms that live in extreme environments, psychrophiles being ones that live in cold environments, and thermophiles being those that live in hot, and when we say hot, we mean temperatures up to and above the boiling point of water.
Now I have to restrain myself, and not hand in an essay of
I've been interested in extremophiles for a while, so I knew there were a lot more types than the above. Writing 1200 words was difficult, as either you write a little about a lot of types, or a lot about a few of the types. Including diagrams was also not easy - there wasn't a lot to draw really.
The question starts off with getting you to define a few terms and come up with definitions for the protein and about transcription factors. There was also mention of luciferase which is a popular marker protein, as it comes from organisms like fireflies and so emits light.
Basically the start of the gene for spectrin had been glued onto the luciferase gene, and then tampered with in various ways, and you had to work out what it all meant. Along the way the GATA transcription factor is introduced and shown to affect the way the protein is produced, and you need to work out why, from graphical and tabular data.
I had to ask my tutor for clarification on one part, as it seemed to just be extracting numbers from a table, and describing a graph - both of which seemed rather simple.
I'd answered the whole question and was fairly happy with it mostly, when it occurred to me that the data we were given probably hadn't been just made up. After a bit of a pubmed search, I found the original paper they must have used for the question, including the same results. It wasn't actually that useful in helping to answer the question. It did make me feel slightly smug though!
As part of this work I also updated various of the wikipedia pages on various of the extremophile types, spectrin and GATA-1, which now includes this paper as a reference.
Q1
Write a report in which you discuss the statement ‘Extremophiles are only psychrophiles or thermophiles’ of 1200 words.For this you have the books, but also a scientific paper was included attached to the question which summarised some of the different extremophiles. Extremophiles are organisms that live in extreme environments, psychrophiles being ones that live in cold environments, and thermophiles being those that live in hot, and when we say hot, we mean temperatures up to and above the boiling point of water.
Now I have to restrain myself, and not hand in an essay of
No. (1 word)
I've been interested in extremophiles for a while, so I knew there were a lot more types than the above. Writing 1200 words was difficult, as either you write a little about a lot of types, or a lot about a few of the types. Including diagrams was also not easy - there wasn't a lot to draw really.
Q2
A question about protein and gene analysis. The protein in question is spectrin, and the transcription factor GATA-1. I hadn't the first idea what to do with this question to start with. However - having conquered my fear a little, and had a longer look at the question, and also used the PDF search facility to locate spectrin in the text, I felt a little more confident.The question starts off with getting you to define a few terms and come up with definitions for the protein and about transcription factors. There was also mention of luciferase which is a popular marker protein, as it comes from organisms like fireflies and so emits light.
Basically the start of the gene for spectrin had been glued onto the luciferase gene, and then tampered with in various ways, and you had to work out what it all meant. Along the way the GATA transcription factor is introduced and shown to affect the way the protein is produced, and you need to work out why, from graphical and tabular data.
I had to ask my tutor for clarification on one part, as it seemed to just be extracting numbers from a table, and describing a graph - both of which seemed rather simple.
I'd answered the whole question and was fairly happy with it mostly, when it occurred to me that the data we were given probably hadn't been just made up. After a bit of a pubmed search, I found the original paper they must have used for the question, including the same results. It wasn't actually that useful in helping to answer the question. It did make me feel slightly smug though!
As part of this work I also updated various of the wikipedia pages on various of the extremophile types, spectrin and GATA-1, which now includes this paper as a reference.
Monday, 2 July 2007
Space, alchemy, chemistry and throwing rocks.
Its another day out with the open university. This time its a day of talks, tutorials and other stuff happening at the Leicester National Space Centre.
We arrived at about 10, for a 10:30 start. The first problem showed up as we got there. I had thought to myself the last thing I should do is forget the piece of paper with the itinerary on it - and sure enough - that was the last thing I did - forget it!
Oh well, CR recognised me when we got to the front of the queue, and they had updated timetables so that was no problem! We also got our tickets to the various lectures.
The first talk was by Professor Barry Jones, and covered some of the block 12 material about extraterrestrial life. He covered a bit about how life might have started and the necessary conditions. He then talked about the chances of life on Mars and Europa. He also talked a bit about exo-planets and the search for earth like ones. I tried to stop myself from thinking "that would have been a good point to include in my ECA". I asked him a couple of question after the talk, as did a few others.
Then we had an S103 tutorial titled "The real Alchemy" on atomic numbers and radioactivity given by Dr Anthony Headley. It showed the difference between nuclear and chemical reactions, and the alchemists dream of changing one element into another. It was followed by a breakout session where we worked through some examples in groups. Things like, what would 14C decay into and what happens to the atomic number and mass.
After a brief lunch, we went to another tutorial on organic chemistry by the same Dr Headley. This was followed by another breakout session where we worked with chemical model kits, making up polymers. Tutors were on hand to help out and check our answers - I think our table made the longest molecule of polythene.
Then another talk by Dr Emma Taylor on crater impact research. She has the use of a very big gun to blast things at other things to try and reproduce craters on moons and similar. She also did practice drops of ball bearings into Sainsburies flour, which is apparently a lot less expensive to set up than a week long test on the big guns!
Finally we went to a talk about rocks from space by Dr Victoria Pearson. This looked at all the types of meteorites that come from space, and how you find them. She had brought along a suitcase full of samples - including a very heavy iron one which we were all much impressed by. Also she had a tiny part of a Martian meteorite, a very tiny lunar meteorite and a slightly bigger earth meteorite (having been blasted off the earth, floated around a bit and then come back into the atmosphere). She also had some very thin sections of meteorites which we could look at through polarising microscopes - from these you could work out some of the mineral content.
The talk was given in a sort of open area in the space centre which was rather noisy and subject to interruptions over the tanoy. However it was extremely good, and we attended the last of three talks she had given that day.
The organisers were a little disappointed that not many S103 students had turned up. I talked to a few people present, and there were a lot of short course students it seemed to me (on a rather unscientific survey!) who were looking to see if S103 would be a course they could do.
I got to look at a few of the 2nd and 3rd level courses I was considering - which was also useful.
We arrived at about 10, for a 10:30 start. The first problem showed up as we got there. I had thought to myself the last thing I should do is forget the piece of paper with the itinerary on it - and sure enough - that was the last thing I did - forget it!
Oh well, CR recognised me when we got to the front of the queue, and they had updated timetables so that was no problem! We also got our tickets to the various lectures.
The first talk was by Professor Barry Jones, and covered some of the block 12 material about extraterrestrial life. He covered a bit about how life might have started and the necessary conditions. He then talked about the chances of life on Mars and Europa. He also talked a bit about exo-planets and the search for earth like ones. I tried to stop myself from thinking "that would have been a good point to include in my ECA". I asked him a couple of question after the talk, as did a few others.
Then we had an S103 tutorial titled "The real Alchemy" on atomic numbers and radioactivity given by Dr Anthony Headley. It showed the difference between nuclear and chemical reactions, and the alchemists dream of changing one element into another. It was followed by a breakout session where we worked through some examples in groups. Things like, what would 14C decay into and what happens to the atomic number and mass.
After a brief lunch, we went to another tutorial on organic chemistry by the same Dr Headley. This was followed by another breakout session where we worked with chemical model kits, making up polymers. Tutors were on hand to help out and check our answers - I think our table made the longest molecule of polythene.
Then another talk by Dr Emma Taylor on crater impact research. She has the use of a very big gun to blast things at other things to try and reproduce craters on moons and similar. She also did practice drops of ball bearings into Sainsburies flour, which is apparently a lot less expensive to set up than a week long test on the big guns!
Finally we went to a talk about rocks from space by Dr Victoria Pearson. This looked at all the types of meteorites that come from space, and how you find them. She had brought along a suitcase full of samples - including a very heavy iron one which we were all much impressed by. Also she had a tiny part of a Martian meteorite, a very tiny lunar meteorite and a slightly bigger earth meteorite (having been blasted off the earth, floated around a bit and then come back into the atmosphere). She also had some very thin sections of meteorites which we could look at through polarising microscopes - from these you could work out some of the mineral content.
The talk was given in a sort of open area in the space centre which was rather noisy and subject to interruptions over the tanoy. However it was extremely good, and we attended the last of three talks she had given that day.
The organisers were a little disappointed that not many S103 students had turned up. I talked to a few people present, and there were a lot of short course students it seemed to me (on a rather unscientific survey!) who were looking to see if S103 would be a course they could do.
I got to look at a few of the 2nd and 3rd level courses I was considering - which was also useful.
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